Dossier MALDI - TOF ■ Oligonucleotide analysis by MALDI - MS
نویسنده
چکیده
Matrix-assisted laser desorption (MALDI) time-offlight (TOF) mass spectrometry (MS) has been successfully used for oligonucleotide sequence confirmation by mass analysis of fragments generated by concentration-dependent exonuclease digestion. This approach is particularly well-suited to the analysis of synthetic DNA due to the relatively short length of the DNA strands. Additionally, an important application of MALDITOF is identification of the extent of failure sequences associated with automated oligonucleotide synthesis.
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